ib m antibacterial peptides against e coli atcc 25922  (ATCC)

Journal: PLOS One

Article Title: Structural and functional analysis of Escherichia coli membrane disruption by Ib-M peptides

doi: 10.1371/journal.pone.0334029

Figure Lengend Snippet: (A-C) E. coli without treatment. (D-F) Ib-M1. ( G-I ) Ib-M2. (J-L) Ib-M6. (M-O) E. coli treated with PMB. The alterations are indicated as follows: large bulges or spherical elements (asterisk), bulges in formation (white arrowhead), collapsed cells (yellow arrowhead), pores (white arrow), invaginations and deep wrinkles (yellow arrow), debris of cell membranes (white circles), and unchanged surface cells (black squares). All images are representative of three biological replicates with similar results.

Article Snippet: Collectively, our findings reveal the possible mechanisms of action of Ib-M antibacterial peptides against E. coli ATCC 25922 and provide strong scientific evidence supporting their therapeutic potential for combating bacterial pathogens.

Techniques:

Journal: PLOS One

Article Title: Structural and functional analysis of Escherichia coli membrane disruption by Ib-M peptides

doi: 10.1371/journal.pone.0334029

Figure Lengend Snippet: (A, F) E. coli without treatment. (B, G) Ib-M1. ( C, H, and I ) Ib-M2. (D, J) Ib-M6. (E, K) E. coli treated with PMB. The alterations are indicated as follows: cytoplasmic retraction (black bracket), bulges (white arrowhead), invagination (yellow arrow), electron-dense material (black arrow and arrowhead), lysed cells (black oval), cytoplasmic vacuoles (thin black arrow), cellular debris-lipid material (black square), and pore (white arrow). All images are representative of three biologically independent assays performed with similar results.

Article Snippet: Collectively, our findings reveal the possible mechanisms of action of Ib-M antibacterial peptides against E. coli ATCC 25922 and provide strong scientific evidence supporting their therapeutic potential for combating bacterial pathogens.

Techniques:

Journal: PLOS One

Article Title: Structural and functional analysis of Escherichia coli membrane disruption by Ib-M peptides

doi: 10.1371/journal.pone.0334029

Figure Lengend Snippet: Interaction of Ib-M peptides with LPS and permeabilization of the E. coli outer membrane. (A) Determination of BODIPY-TR cadaverine displacement from LPS by Ib-M peptides after 60 min of exposure. PMB was utilized as a positive control. Values are expressed as mean ± SD of three independent assays. Statistical analysis was performed using ANOVA with Tukey’s multiple comparison test. (B) NPN fluorescence measurement upon exposure of E. coli ATCC 25922 to Ib-M1 peptide. PMB was used as the positive control at a concentration of 12.5 µM. The negative control was comprised of bacteria without the addition of peptides. Measurements were obtained at minute 60. The mean ± SD of a representative assay is presented. Significant differences were established and are denoted as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns indicates no significance.

Article Snippet: Collectively, our findings reveal the possible mechanisms of action of Ib-M antibacterial peptides against E. coli ATCC 25922 and provide strong scientific evidence supporting their therapeutic potential for combating bacterial pathogens.

Techniques: Membrane, Positive Control, Comparison, Fluorescence, Concentration Assay, Negative Control, Bacteria

Journal: PLOS One

Article Title: Structural and functional analysis of Escherichia coli membrane disruption by Ib-M peptides

doi: 10.1371/journal.pone.0334029

Figure Lengend Snippet: Inner permeabilization of E. coli ML35. ( A ) Ib-M1. ( B ) Ib-M2. ( C ) Ib-M6. Measurements were obtained every five min for 8 h. PBS without the addition of ONPG served as the negative control. ( D ) PMB and ( E ) streptomycin were employed as the positive and negative controls, respectively. Time-dependent cytoplasmic membrane depolarization of E. coli ATCC25922 induced by ( F ) Ib-M1, ( G ) Ib-M2, and ( H ) Ib-M6; as determined using the membrane potential-sensitive fluorescent dye, DiSC 3 . Dye release was monitored by measuring fluorescence at an excitation wavelength of 620 nm and an emission wavelength of 670 nm. Triton X-100 was used as the positive control. Data are expressed as mean ± SD of three independent assays.

Article Snippet: Collectively, our findings reveal the possible mechanisms of action of Ib-M antibacterial peptides against E. coli ATCC 25922 and provide strong scientific evidence supporting their therapeutic potential for combating bacterial pathogens.

Techniques: Negative Control, Membrane, Fluorescence, Positive Control